Johns Hopkins Orthopaedic Surgery
February 3, 2014
The new theory, reported in Nature Medicine, suggests that initial harm to the cartilage causes the bone underneath it to build surplus bone that in turn stretches the cartilage above and speeds its decline.
“We began to think of cartilage and subchondral bone as functioning as a single unit,” saysXu Cao, director of the Center for Musculoskeletal Research in the Department of Orthopaedic Surgery. “That helped us to see the ways in which the bone was responding to changes in the cartilage and exacerbating the problem.”
Using mice with anterior cruciate ligament tears, the researchers found that as soon as one week after the injury, pockets of subchondral bone had been “chewed” away by cells called osteoclasts. This process activated high levels in the bone of a protein called TGF-beta1, which recruited stem cells to the site so that they could create new bone to fill the holes. Cao calls these pockets of new bone formation “osteoid islets.”
But the bone building and the bone destruction processes were not coordinated in the mice, and the bone building prevailed, placing further strain on the cartilage cap. It is this extraneous bone formation that Cao and his colleagues believe to be at the heart of osteoarthritis, as confirmed in a computer simulation of the human knee.
The team then tried several methods to block the activity of TGF-beta1. When a TGF-beta1 inhibitor drug was given intravenously, the subchondral bone improved significantly, but the cartilage cap deteriorated further. However, when a different inhibitor of TGF-beta1, an antibody against it, was injected directly into the subchondral bone, the positive effects were seen in the bone without the negative effects on the cartilage. The same result was also seen when TGF-beta1 was genetically disrupted in the bone precursor cells alone.
“Our results are potentially really good news for patients,” says Cao. “We are already working to develop a clinical trial to test the efficacy of locally applied TGF-beta1 antibodies in human patients at early stages of the disease.” If successful, their nonsurgical treatment could make osteoarthritis halt in its tracks, he says.